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Production of carotenoids grown on waste fat
Holub, Jiří ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Carotenoids are lipid-soluble pigments which are contained in plants and microorganisms. Carotenoids are known for their antioxidant effects. Lipids are predominantly non-polar substances, which are essential and present in all types of organisms. The research was made by theoretical and practical form. It was dealing with production of carotenoids pigments and different lipophilic substances by yeasts of Rhodotorula glutinis, Rhodosporidium toruloides and Sporidiobolus metaroseus, cultivated in media containing waste fat or glycerol. Further, methods for obtaining lipid substances from microorganisms and with further analysis of samples by gas or liquid chromatography were optimized. The main task of the research was to find out which types of media and yeasts are the most suitable for production of lipid substances. For production of lipid substances in glycerol media, as the best producing strain Rhodosporidium toruloides was found, which simultaneously reported the highest concentrations of carotenoids on fatty and fatty hydrolyzed media.
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Deactivation of the Intramolecular Charge Transfer state in RC-LH1 complexes of genetically modified purple bacteria \kur{Rhodobacter sphaeroides}
ŠÍMOVÁ, Ivana
The main task of this thesis is to find the ICT activator of a carotenoid spheroidenone in the pigment-protein complex LH1 of Rhodobacter sphaeroides. The ICT state is characterized by a red-shifted positive transient absorption signal around 750 - 800 nm. We hypothesize that the presence of the ICT state is associated with the pigment-protein interaction of a carotenoid keto group and specific amino acid in the protein. To perform the experiment, four mutant complexes with amino acids substitutions, which should result in decreased intensity of the ICT signal due to a weaker or missing pigment-protein interaction, were obtained and measured using femtosecond time resolved pump-probe spectroscopy.
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Production of carotenoids grown on waste fat
Holub, Jiří ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Carotenoids are lipid-soluble pigments which are contained in plants and microorganisms. Carotenoids are known for their antioxidant effects. Lipids are predominantly non-polar substances, which are essential and present in all types of organisms. The research was made by theoretical and practical form. It was dealing with production of carotenoids pigments and different lipophilic substances by yeasts of Rhodotorula glutinis, Rhodosporidium toruloides and Sporidiobolus metaroseus, cultivated in media containing waste fat or glycerol. Further, methods for obtaining lipid substances from microorganisms and with further analysis of samples by gas or liquid chromatography were optimized. The main task of the research was to find out which types of media and yeasts are the most suitable for production of lipid substances. For production of lipid substances in glycerol media, as the best producing strain Rhodosporidium toruloides was found, which simultaneously reported the highest concentrations of carotenoids on fatty and fatty hydrolyzed media.
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Protein Control Over Carotenoid Spectroscopy and Functions
ŠLOUF, Václav
The photophysics of pigments is influenced, to an extent depending on its structure, by the properties of the environment. Proteins represent a very specific environment at least in two aspects: i) they are native to most of the pigments in living systems; ii) they facilitate modifications of pigment configuration, leading to changes not only in its spectroscopic properties, but also in its functional abilities. In studies presented in this thesis, femtosecond pump-probe spectroscopy was used to study predominantly the photosynthetic antenna complexes of bacteria and algae. Based on spectroscopic evidence, the structural modifications of pigments imposed by the protein were deduced or hypothesized, together with their functional relevance.
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